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KMID : 0357920130470060507
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Korean Journal of Pathology
2013 Volume.47 No. 6 p.507 ~ p.518
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Endogenous Gastric-Resident Mesenchymal Stem Cells Contribute to Formation of Cancer Stroma and Progression of Gastric Cancer
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Kim Eun-Kyung
Kim Hye-Jung
Yang Young-Il
Kim Jong-Tae
Choi Min-Young
Choi Chang-Soo
Kim Kwang-Hee
Lee Jeong-Han
Jang Won-Hee
Cheong Soon-Ho
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Abstract
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Background: Carcinoma-associated fibroblasts (CAFs) contribute to carcinogenesis and cancer progression, although their origin and role remain unclear. We recently identified and investigated the in situ identity and implications of gastric submucosa-resident mesenchymal stem cells (GSMSCs) in the progression of gastric carcinogenesis.
Methods: We isolated GS-MSCs from gastric submucosa using hydrogel-supported organ culture and defined their identity. Isolated cells were assessed in vitro by immunophenotype and mesengenic multipotency. Reciprocal interactions between GS-MSCs and gastric cancer cells were evaluated. To determine the role of GS-MSCs, xenografts were constructed of gastric cancer cells admixed with or without GS-MSCs.
Results: Isolated cells fulfilled MSCs requirements in regard to plastic adherence, stromal cell immunophenotype, and multipotency. We demonstrated a paracrine loop that gastric cancer cells enhanced the migration, proliferation, and differentiation of GS-MSCs; additionally, GS-MSCs promoted the proliferation of gastric cancer cell in vitro. Xenograft experiments showed that GSMSCs significantly promoted cancer growth and angiogenesis. GS-MSCs that integrated into gastric cancer became not only CAFs but also rarely endothelial cells which contributed to the formation of cellular and vascular cancer stroma.
Conclusions: Endogenous GS-MSCs play an important role in gastric cancer progression.
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KEYWORD
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Gastric-resident mesenchymal stem cell, Carcinoma-associated fibroblast, Cancer stroma, Stomach neoplasms
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